Anti-ASK1 Rabbit pAb

Anti-ASK1 Rabbit pAb

Antibodies Oncology
AO-03-GB114120-100
114120,MAPK/ERK kinase kinase 5,MAP3K5,GB114120,GB114120100,ASK1,MEKK5,ASK 1,MEKK 5,ASK-1,MAPKKK5,MEK kinase 5,Mitogen-activated protein kinase kinase kinase 5
check En Stock
Hurry! only 10 items left in stock.
429,00 €
HT
Quantité

Ajouter souhaits

Product Information


Protein full name

Mitogen-activated protein kinase kinase kinase 5

Synonyms

ASK-1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

Uniprot ID

Q99683

Immunogen

KLH conjugated Synthetic peptide corresponding to Human ASK1

Isotype

IgG

Predicted MW./Observed MW.

155 kDa / 155 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 1000 A431, Jurkat, Caco-2



Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS).



Images

01O44SeLUqJULw7.jpg

 WB analysis of ASK1(GB114120).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB114120-100
: 10 Produits
Hurry! only 10 items left in stock.

Use collapsible tabs for more detailed information that will help customers make a purchasing decision.

Ex: Shipping and return policies, size guides, and other common questions.

  • Paste the label on a flat surface on the package
  • Make sure that both 1D and 2D barcodes are clearly visible
  • Ensure that the label is smooth and isn’t creased or wrinkled
  • Check for any tears, dents, holes or scratches
  • Pack your product tightly, with the right size packaging
  • Ensure both barcodes are on a flat surface of the package