Anti-Cyclophilin B Rabbit pAb

Anti-Cyclophilin B Rabbit pAb

Antibodies Oncology
AO-03-GB113330-100
Cyclophilin B,GB113330100,113330,S cyclophilin,PPIB,PPIase B,CYP S1,CyclophilinB,GB113330,SCYLP,CB,CYPB,Cyclophilin-B,Rotamase B,Peptidyl-prolyl cis-trans isomerase B
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Product Information


Protein full name

Peptidyl-prolyl cis-trans isomerase B

Synonyms

Cyclophilin B, CYP S1, CYPB, PPIase B, PPIB, Rotamase B, S cyclophilin, SCYLP

Uniprot ID

P24369

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cyclophilin B

Isotype

IgG

Predicted MW./Observed MW.

24 kDa / 18 kDa

Purity

Affinity purification

Subcellular location

Endoplasmic reticulum



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 brain, kidney, liver
IHC/IF Mouse, Rat 1: 1500-1: 3000 brain



Background

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).



Images

01anShQ55CZ4fRc.jpg

 WB analysis of Cyclophilin B(GB113330).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

029t1rO7WkkNsNF.jpg

 IHC analysis of Cyclophilin B(GB113330).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03EbEb6g65GFSG8.jpg

 IHC analysis of Cyclophilin B(GB113330).
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB113330-100
: 10 Produits
Hurry! only 10 items left in stock.

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