Anti-PHAPI2/APRIL Rabbit pAb

Anti-PHAPI2/APRIL Rabbit pAb

Antibodies Oncology
AO-03-GB112911-100
112911,PHAPI2 / APRIL,Ssp29,A,PHAPI2,PHAPI2a,PAL 31,GB112911,Anp32b,Pal31,GB112911100,Proliferation-related acidic leucine-rich protein PAL31,PHAPI-2,PHAPI 2,Acidic leucine-rich nuclear phosphoprotein 32 family member B
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Product Information


Protein full name

Acidic leucine-rich nuclear phosphoprotein 32 family member B

Synonyms

Proliferation-related acidic leucine-rich protein PAL31, Anp32b, Pal31, PAL 31, Ssp29, PHAPI2a

Uniprot ID

Q9EST5

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse PHAPI2 / APRIL

Isotype

IgG

Predicted MW./Observed MW.

31 kDa / 31 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 1000 Jurkat
IHC/IF Mouse, Rat 1: 300-1: 1200 spleen, heart, kidney



Background

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).



Images

01CxlWIrqDiBFNz.jpg

 WB analysis of PHAPI2/APRIL(GB112911).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02h9qSv1XwQhQb6.jpg

 IF analysis of PHAPI2/APRIL(GB112911)red.
Sample: Rat prostate gland, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

03qD0KKZBtKBDkE.jpg

 IF analysis of PHAPI2/APRIL(GB112911)red.
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112911-100
: 10 Produits
Hurry! only 10 items left in stock.

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