Anti-CLIC4 Rabbit pAb

Anti-CLIC4 Rabbit pAb

Antibodies Oncology
AO-03-GB112388-100
CLIC4,Clic4,GB112388100,p64H1,112388,GB112388,Intracellular chloride ion channel protein p64H1,CLIC 4,CLIC-4,Anti- CLIC4 Rabbit pAb,mtCLIC,mc3s5/mtCLIC,Chloride intracellular channel protein 4
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Product Information


Protein full name

Chloride intracellular channel protein 4

Synonyms

mc3s5/mtCLIC, Clic4, Intracellular chloride ion channel protein p64H1, mtCLIC, p64H1

Uniprot ID

Q9QYB1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse CLIC4

Isotype

IgG

Predicted MW./Observed MW.

29 kDa / 29 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Cell membrane



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 400-1: 800 kidney, ovary
ICC/IF Human 1: 50-1: 200 HeLa



Background

Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis.



Images

01dFaONexRkMmjQ.jpg

 WB analysis of CLIC4(GB112388).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02CXYN3JRum9F7Z.jpg

 ICC analysis of CLIC4(GB112388)green.
Sample: Hela, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 50, 4℃ overnight.
Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112388-100
: 10 Produits
Hurry! only 10 items left in stock.

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