Anti-Bcl 7A Rabbit pAb
   

Anti-Bcl 7A Rabbit pAb

Antibodies Oncology
AO-03-GB112383-100
B cell CLL/lymphoma 7,Anti- Bcl 7A Rabbit pAb,B-7,Bcl7A,Bcl7a,112383,Bcl 7A,GB112383,BCL7,B 7,GB112383100,B7,B cell CLL/lymphoma 7A,Bcl-7A,B-cell CLL/lymphoma 7 protein family member A
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Ajouter souhaits

Product Information


Protein full name

B-cell CLL/lymphoma 7 protein family member A

Synonyms

Bcl7a, BCL7, B cell CLL/lymphoma 7A, B cell CLL/lymphoma 7

Uniprot ID

Q4VC05

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Bcl 7A

Isotype

IgG

Predicted MW./Observed MW.

23 kDa / 38 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 250-1: 500 293T
IHC/IF Mouse, Rat 1: 400-1: 800 cerebellum, hippocampus, prefrontal lobe



Background

This gene is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene.



Images

01Fdkqp1hAgIDNq.jpg

 WB analysis of Bcl 7A(GB112383).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02xwXyTOsOTBx1l.jpg

 IF analysis of Bcl 7A(GB112383)red.
Sample: Mouse cerebellum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

03fIFxoBtGaCAlU.jpg

 IF analysis of Bcl 7A(GB112383)red.
Sample: Mouse prefrontal lobe, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

04m8uLv2dR56zTx.jpg

 IF analysis of Bcl 7A(GB112383)red.
Sample: Rat cerebellum, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

05Tgn387NtScjoq.jpg

 IF analysis of Bcl 7A(GB112383)red.
Sample: Rat hippocampus, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112383-100
: 10 Produits
Hurry! only 10 items left in stock.

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