Anti-cIAP1 Rabbit pAb
   

Anti-cIAP1 Rabbit pAb

Antibodies Oncology
AO-03-GB112300-100
GB112300,GB112300100,112300,cIAP-1,Cellular inhibitor of apoptosis 1,cIAP 1,Anti- cIAP1 Rabbit pAb,cIAP1,mIAP2,Inhibitor of apoptosis protein 2,C-IAP1,Birc2,RING-type E3 ubiquitin transferase BIRC2,Baculoviral IAP repeat-containing protein 2
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Ajouter souhaits

Product Information


Protein full name

Baculoviral IAP repeat-containing protein 2

Synonyms

Cellular inhibitor of apoptosis 1, C-IAP1, Inhibitor of apoptosis protein 2, mIAP2, RING-type E3 ubiquitin transferase BIRC2, Birc2

Uniprot ID

Q62210

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse cIAP1

Isotype

IgG

Predicted MW./Observed MW.

70 kDa / 70 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 1000-1: 2000 spleen, testis
IHC/IF Human, Mouse, Rat 1: 700-1: 4000 brain, colon, heart



Background

Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).



Images

01pfdMkh9wDOCzO.jpg

 WB analysis of cIAP1(GB112300).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02z23FEqG5QDCnq.jpg

 IHC analysis of cIAP1(GB112300).
Sample: Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 2100, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03K76fSOd33t12R.jpg

 IHC analysis of cIAP1(GB112300).
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 2100, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04a0hdSOGK0xGdv.jpg

 IHC analysis of cIAP1(GB112300).
Sample: Rat uterus, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 2100, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

0514Hft1uA5bpPl.jpg

 IF analysis of cIAP1(GB112300)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

06yJgoeGWHGQpY5.jpg

 IF analysis of cIAP1(GB112300)red.
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB112300-100
: 10 Produits
Hurry! only 10 items left in stock.

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