Anti-ZIP Kinase Rabbit pAb

AO-03-GB111907-100
Dlk,ZIP kinase,ZIPKinase,GB111907100,Zipk,ZIP,GB111907,ZIP Kinase,DAP-like kinase,ZK,ZIP-Kinase,DAP kinase 3,111907,MYPT1 kinase,Dapk3,Death-associated protein kinase 3
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Product Information


Protein full name

Death-associated protein kinase 3

Synonyms

DAP kinase 3, DAP-like kinase, Dlk, MYPT1 kinase, ZIP kinase, Dapk3, Zipk, ZIP

Uniprot ID

O54784

Immunogen

Recombinant protein corresponding to Mouse ZIP Kinase

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm




Applications


ApplicationsSpeciesDilutionPositive Tissue
IHC/IFMouse1: 500-1: 2000brain, small intestine




Background


DAPK3(Death-associated protein kinase 3) is also named as ZIPK(Zipper-interacting protein kinase),Dlk(DAP-like kinase). It is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain (RLC) to promote membrane blebbing. DAPK3 mRNA and protein are abundant in heart tissue and isolated ventricular neonatal rat cardiac myocytes. It has 2 isoforms produced by alternative splicing,named as ZIPK-L and ZIPK-S. This antibody is specific to ZIPK-Phospho 311Ser.




Images

01Wk54pKHXQYVIF.jpg                        

IHC analysis of ZIP Kinase(GB111907).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02CJEXVw2A1yAmE.jpg                        

IHC analysis of ZIP Kinase(GB111907).
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.




Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).


: AO-03-GB111907-100
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Hurry! only 10 items left in stock.

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