Product Information
Protein full name | Death-associated protein kinase 3 |
Synonyms | DAP kinase 3, DAP-like kinase, Dlk, MYPT1 kinase, ZIP kinase, Dapk3, Zipk, ZIP |
Uniprot ID | O54784 |
Immunogen | Recombinant protein corresponding to Mouse ZIP Kinase |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Nucleus, Cytoplasm |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Mouse | 1: 500-1: 2000 | brain, small intestine |
Background
DAPK3(Death-associated protein kinase 3) is also named as ZIPK(Zipper-interacting protein kinase),Dlk(DAP-like kinase). It is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain (RLC) to promote membrane blebbing. DAPK3 mRNA and protein are abundant in heart tissue and isolated ventricular neonatal rat cardiac myocytes. It has 2 isoforms produced by alternative splicing,named as ZIPK-L and ZIPK-S. This antibody is specific to ZIPK-Phospho 311Ser.
Images
| IHC analysis of ZIP Kinase(GB111907). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of ZIP Kinase(GB111907). Sample: Mouse small intestine, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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