Product Information
Protein full name | Tumor necrosis factor ligand superfamily member 12 |
Synonyms | TNF-related weak inducer of apoptosis, TWEAK, Tnfsf12, APO3 ligand, DR3LG, TNFSF12 protein, APO3/DR3 ligand |
Uniprot ID | O54907 |
Immunogen | Recombinant protein corresponding to Mouse TWEAK |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | Cell membrane, Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Mouse | 1: 500-1: 2000 | brain |
Background
TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis.
Images
| IHC analysis of TWEAK(GB111772). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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