Anti-DFFB/CAD Rabbit pAb

Anti-DFFB/CAD Rabbit pAb

Antibodies Oncology
AO-03-GB111721-100
Dffb,111721,DNA fragmentation factor 40 kDa subunit,CPAN,GB111721100,Anti-DFFB/CAD Rabbit pAb,CAD,GB111721,Cad,DFFB/CAD,Caspase-activated DNase,DFF-40,Caspase-activated deoxyribonuclease,DNA fragmentation factor subunit beta
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Product Information


Protein full name

DNA fragmentation factor subunit beta

Synonyms

Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, DNA fragmentation factor 40 kDa subunit, DFF-40, Dffb, Cad, CPAN

Uniprot ID

O54788

Immunogen

Recombinant protein corresponding to Mouse DFFB/CAD

Isotype

IgG

Predicted MW./Observed MW.

39 kDa / 39 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 kidney, thymus, colon



Background

Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. CAD, which can be activated by caspases, exists as a complex with its inhibitor, inhibitor of CAD (ICAD). ICAD helps correct folding of CAD and remains complexed with CAD to inhibit the DNase activity of CAD. Caspase, which is activated during the apoptotic process, cleaves ICAD, and the CAD released from ICAD enters the nuclei to cleave the chromosomal DNA.



Images

015MUI4vjJsNxj5.jpg

 WB analysis of DFFB/CAD(GB111721).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02dvgw1l75ayQU4.jpg

 WB analysis of DFFB/CAD(GB111721).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03XvEeRpukR3aJ8.jpg

 WB analysis of DFFB/CAD(GB111721).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111721-100
: 10 Produits
Hurry! only 10 items left in stock.

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