Product Information
|
Protein full name |
Protein AATF |
|
Synonyms |
Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1, Traube protein, Aatf, Che1, Trb, Rb binding protein Che 1 |
|
Uniprot ID |
Q9JKX4 |
|
Immunogen |
Recombinant protein corresponding to Mouse AATF |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
59 kDa / 63 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, kidney, thymus |
Background
The gene may function as a general inhibitor of the histone deacetylase HDAC1.The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis.
Images
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WB analysis of AATF/DED(GB111594). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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|
WB analysis of AATF/DED(GB111594). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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