Anti-Cyclophilin F Rabbit pAb

Anti-Cyclophilin F Rabbit pAb

Antibodies Oncology
AO-03-GB111454-100
Ppif,Cyclophilin F,Rotamase F,CF,Cyclophilin D,CyclophilinF,CypD,PPIase F,GB111454100,GB111454,Cyclophilin-F,CyP-D,111454,Anti-Cyclophilin F Rabbit pAb,Peptidyl-prolyl cis-trans isomerase F,mitochondrial
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Product Information


Protein full name

Peptidyl-prolyl cis-trans isomerase F, mitochondrial

Synonyms

PPIase F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Rotamase F, Ppif

Uniprot ID

Q99KR7

Immunogen

Recombinant protein corresponding to Mouse Cyclophilin F

Isotype

IgG

Predicted MW./Observed MW.

22 kDa / 17 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 500-1: 1000 heart, liver, brain



Background

PPIF, also named as CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF.



Images

01HIZqsaSa4d9Fz.jpg

 WB analysis of Cyclophilin F(GB111454).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111454-100
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