Anti-cIAP2 Rabbit pAb
   

Anti-cIAP2 Rabbit pAb

Antibodies Oncology
AO-03-GB111051-100
Cellular inhibitor of apoptosis 2,Inhibitor of apoptosis protein 1,111051,cIAP2,GB111051,GB111051100,C-IAP2,mIAP1,Birc3,cIAP 2,RING-type E3 ubiquitin transferase BIRC3,cIAP-2,Baculoviral IAP repeat-containing protein 3
check En Stock
Hurry! only 10 items left in stock.
429,00 €
HT
Quantité

Ajouter souhaits

Product Information


Protein full name

Baculoviral IAP repeat-containing protein 3

Synonyms

Cellular inhibitor of apoptosis 2, C-IAP2, Birc3, Inhibitor of apoptosis protein 1, mIAP1, RING-type E3 ubiquitin transferase BIRC3

Uniprot ID

O08863

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse cIAP2

Isotype

IgG

Predicted MW./Observed MW.

67 kDa / 67 kDa

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 500-1: 1000 thymus, testis, lymph node
IHC/IF Mouse, Rat 1: 750-1: 3000 lung, lymph node, spleen



Background

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling.



Images

01Z8dVxJYImgJ3e.jpg

 WB analysis of cIAP2(GB111051).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02f2nCFzegolezo.jpg

 IHC analysis of cIAP2(GB111051).
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03x2GWRB0yLYZpw.jpg

 IHC analysis of cIAP2(GB111051).
Sample: Mouse lymph node, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04OHGXga5LJTZRp.jpg

 IHC analysis of cIAP2(GB111051).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05QQY0AOkQgu0sq.jpg

 IHC analysis of cIAP2(GB111051).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

0660lhz52wr2zpX.jpg

 IF analysis of cIAP2(GB111051)red.
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07vIq3kPRNN2C3Z.jpg

 IF analysis of cIAP2(GB111051)red.
Sample: Mouse lymph node, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

080YjK9XX3Yddxb.jpg

 IF analysis of cIAP2(GB111051)red.
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB111051-100
: 10 Produits
Hurry! only 10 items left in stock.

Use collapsible tabs for more detailed information that will help customers make a purchasing decision.

Ex: Shipping and return policies, size guides, and other common questions.

  • Paste the label on a flat surface on the package
  • Make sure that both 1D and 2D barcodes are clearly visible
  • Ensure that the label is smooth and isn’t creased or wrinkled
  • Check for any tears, dents, holes or scratches
  • Pack your product tightly, with the right size packaging
  • Ensure both barcodes are on a flat surface of the package