Product Information
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Protein full name |
Bcl2-associated agonist of cell death |
|
Synonyms |
BAD, BBC2, BBC6, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, BCL2L8, Bcl2 antagonist of cell death, Bcl-XL/Bcl-2-Associated Death Promoter |
|
Uniprot ID |
Q61337 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Bad |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Mitochondrion outer membrane, Cytoplasm |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Human, Mouse, Rat | 1: 300-1: 600 | brain, lung, spleen |
Background
BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Images
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IHC analysis of Bad(GB11198). Sample: Rat brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IF analysis of Bad(GB11198)red. Sample: Rat brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
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IF analysis of Bad(GB11198)red. Sample: Rat lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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