Anti-Survivin Rabbit pAb
   

Anti-Survivin Rabbit pAb

Antibodies Oncology
AO-03-GB11177-100
Anti-Survivin Rabbit pAb,11177,BIRC5,GB11177,SURVIVIN,EPR 1,API4,Survivin,Apoptosis inhibitor 4,IAP4,Apoptosis inhibitor survivin,GB11177100,Baculoviral IAP repeat-containing protein 5
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Product Information


Protein full name

Baculoviral IAP repeat-containing protein 5

Synonyms

API4, Apoptosis inhibitor 4, Apoptosis inhibitor

Uniprot ID

O70201

Immunogen

Recombinant protein corresponding to Mouse Survivin

Isotype

IgG

Predicted MW./Observed MW.

16 kDa / 16 kDa

Purity

Affinity purification

Subcellular location

Spindle, Nucleus, Cytoplasm,



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 600-1: 800 colon, testis, skin
IHC/IF Mouse, Rat 1: 400-1: 800 lung, lung+LPS, spleen



Background

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules.



Images

01tSukMJL3eRFca.jpg

 WB analysis of Survivin(GB11177).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02u7vSA0EvvAQ77.jpg

 IHC analysis of Survivin(GB11177).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03XPsVh7mnM727n.jpg

 IHC analysis of Survivin(GB11177).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 400, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04xuOoIp9OoA791.jpg

 IF analysis of Survivin(GB11177)red.
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

05WrpgV399JEYTN.jpg

 IF analysis of Survivin(GB11177)red.
Sample: Mouse lung+LPS, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11177-100
: 10 Produits
Hurry! only 10 items left in stock.

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