Anti-Raf1 Rabbit pAb

Anti-Raf1 Rabbit pAb

Antibodies Oncology
AO-03-GB11543-100
NS5,RAF1,11543,CMD1NN,CRAF,Raf1,GB11543,GB11543100,Raf-1 proto-oncogene,Raf-1,Raf 1,serine/threonine kinase,c-,RAF proto-oncogene serine/threonine-protein kinase
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Product Information


Protein full name

RAF proto-oncogene serine/threonine-protein kinase

Synonyms

RAF1, Raf-1 proto-oncogene, Serine/threonine kinase, CMD1NN, CRAF, Proto-oncogene c-RAF, NS5, Raf-1, C-Raf, Raf1

Uniprot ID

Q99N57

Immunogen

Recombinant protein corresponding to Mouse c-Raf

Isotype

IgG

Predicted MW./Observed MW.

73 kDa / 73 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 lung, kidney, testis



Background

RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF or simply c-Raf or even Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. The c-Raf protein is part of the ERK1/2 pathway as a MAP kinase kinase kinase (MAP3K) that functions downstream of the Ras subfamily of membrane associated GTPases. C-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases, from the TKL (Tyrosine-kinase-like) group of kinases.



Images

01REqlJuHrUl3Ct.jpg

 WB analysis of Raf1(GB11543).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02hbkefsH33lQmh.jpg

 WB analysis of Raf1(GB11543).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11543-100
: 10 Produits
Hurry! only 10 items left in stock.

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