Anti-Bag3 Rabbit pAb

Anti-Bag3 Rabbit pAb

Antibodies Oncology
AO-03-GB11536-100
GB11536100,BAG3,Bag 3,MFM6,BCL2 associated athanogene 3,BAG-3,Docking protein CAIR-1,Bag-3,11536,BIS,GB11536,Bag3,CAIR-1,BAG family molecular chaperone regulator 3
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Product Information


Protein full name

BAG family molecular chaperone regulator 3

Synonyms

BAG3, BAG-3, BIS, CAIR-1, MFM6, BCL2

Uniprot ID

O95817

Immunogen

Recombinant protein corresponding to Human Bag3

Isotype

IgG

Predicted MW./Observed MW.

62 kDa / 86 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 heart, muscle, brain



Background

BAG proteins compete with Hip-1 for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.



Images

01imlL2YjmkBnJX.jpg

 WB analysis of Bag3(GB11536).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02bGWG0Y7i2Q6Q3.jpg

 WB analysis of Bag3(GB11536).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11536-100
: 10 Produits
Hurry! only 10 items left in stock.

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