Anti-Cleaved-Caspase-3 Rabbit pAb
   

Anti-Cleaved-Caspase-3 Rabbit pAb

Antibodies Oncology
AO-03-GB11532-100
Caspase 3,Caspase3,caspase3,GB11532100,GB11532,PARP cleavage protease,Caspase-3,CC,CASP3,CPP32,SCA-1,11532,Caspase-3 subunit p12,Cleaved- Caspase-3,Cleaved,Cleaved Caspase 3,c caspase 3,CPP32B,c-caspase 3,CleavedCaspase3,casepase,Caspase-3,Cleaved-caspase3,cleaved Caspase-3,c-caspase3,Cleaved Caspase3,capase,C-Caspase-3,c-caspase,capase3
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Ajouter souhaits

Product Information


Protein full name

Caspase-3

Synonyms

CASP3, CPP32, CPP32B, SCA-1, caspase 3

Uniprot ID

P70677

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Caspase-3

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human, Mouse, Rat 1: 500-1: 1000 colon cancer, esophagus cancer, spleen
ICC/IF Human, Mouse, Rat 1: 500-1: 1000 C212, HeLa, L6



Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity).



Images

01Drws04xR3XWd3.jpg

 IHC analysis of Cleaved-Caspase-3(GB11532).
Sample: Human subcutaneous transplant tumor, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02HRaVtzKTefg5C.jpg

 IHC analysis of Cleaved-Caspase-3(GB11532).
Sample: Human stomcah cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03kYqqOAMOBtsCb.jpg

 IHC analysis of Cleaved-Caspase-3(GB11532).
Sample: Mouse subcutaneous transplant tumor, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04KjVYU36uPKDnE.jpg

 IHC analysis of Cleaved-Caspase-3(GB11532).
Sample: Rat spleen+LPS, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05ahhHadd0874Zx.jpg

 IF analysis ofCleaved-Caspase-3(GB11532)red.
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

067mh2jeXEUuXKL.jpg

 IF analysis ofCleaved-Caspase-3(GB11532)red.
Sample: Human subcutaneous transplant tumor K562, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07aTsLia84MTh0J.jpg

 IF analysis ofCleaved-Caspase-3(GB11532)red.
Sample: Human stomcah cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08JDfU2dNCUNomL.jpg

 IF analysis ofCleaved-Caspase-3(GB11532)red.
Sample: Human esophagus cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

09LaSp8Fuo8ItNQ.jpg

 IF analysis ofCleaved-Caspase-3(GB11532)red.
Sample: Human subcutaneous transplant tumor, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

10NCXuAh34cYyWb.jpg

 IF analysis ofCleaved-Caspase-3(GB11532)red.
Sample: Human subcutaneous transplant tumor, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

11Es3YPRfPC7TRt.jpg

 IF analysis ofCleaved-Caspase-3(GB11532)red.
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

12JNi09vTYcInHF.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: C2C12 Stau 0h, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

13dkoLUpGxgAKhq.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: C2C12 Stau 6h, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

14uELjkbPfIhTwA.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: HeLa Stau 0h, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

15kf4dTTaQaUxhb.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: HeLa Stau 3h, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

16BRJAkyeqlOqhm.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: HeLa Stau 6h, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

17j0eF6aGkFPbTP.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: L6 Stau 0h, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

188c0PWmTh0Al4L.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: L6 Stau 3h, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

1928GO7xdQhtK1D.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: L6 Stau 6h, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

20rWo6r50Jz9Prh.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: C2C12, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

212ElYKIfLKYwYh.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: HeLa, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

22Tgl6PnUiCdbGg.jpg

 ICC analysis of Cleaved-Caspase-3(GB11532)red.
Sample: L6, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11532-100
: 10 Produits
Hurry! only 10 items left in stock.

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