Anti-AIF Rabbit pAb
   

Anti-AIF Rabbit pAb

Antibodies Oncology
AO-03-GB11314-100
NAMSD,CMT2D,DFNX5,NADMR,GB11314100,apoptosis inducing factor,GB11314,COXPD6,COWCK,apoptosis inducing factor mitochondria associated 1,mitochondria associated 1,CMTX4,AIF,Anti-AIF Rabbit pAb,11314,AIFM1,PDCD8,Apoptosis-inducing factor 1,mitochondrial
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Product Information


Protein full name

Apoptosis-inducing factor 1, mitochondrial

Synonyms

AIFM1, AIF, CMT2D, CMTX4, COWCK, COXPD6, NADMR, NAMSD, PDCD8, DFNX5, apoptosis inducing factor, mitochondria associated 1, apoptosis inducing factor mitochondria associated 1

Uniprot ID

Q9Z0X1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse AIF

Isotype

IgG

Purity

Affinity purification

Subcellular location

Mitochondrion intermembrane space, Mitochondrion inner membrane, Cytoplasm, Nucleus



Applications


Applications Species Dilution Positive Tissue
IHC/IF Human, Mouse, Rat 1: 500-1: 2000 heart, Skeletal muscle, spleen



Background

Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA.



Images

01amvKnVtxVR7Mf.jpg

 IHC analysis of AIF(GB11314).
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02pp2YKrCHfJsu2.jpg

 IHC analysis of AIF(GB11314).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03WSTTUys6mtoaH.jpg

 IHC analysis of AIF(GB11314).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04tnI2DXSs4jpBv.jpg

 IHC analysis of AIF(GB11314).
Sample: Rat thymus, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05hl78l235XrNz1.jpg

 IF analysis of AIF(GB11314)red.
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

06YgS2ilhVgnrez.jpg

 IF analysis of AIF(GB11314)red.
Sample: Mouse Skeletal muscle, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07hrzBcDUYjBUGn.jpg

 IF analysis of AIF(GB11314)red.
Sample: Mouse Skeletal muscle, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08DlTF9nET6LipH.jpg

 IF analysis of AIF(GB11314)red.
Sample: Rat Skeletal muscle, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11314-100
: 10 Produits
Hurry! only 10 items left in stock.

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