Anti-Shf Rabbit pAb
   

Anti-Shf Rabbit pAb

Antibodies Oncology
AO-03-GB11688-100
GB11688,GB11688100,Shb-like adapter protein,Src homology 2 domain containing F,Shf,11688,SH2 domain-containing adapter protein F,SH2 domain-containing adapter protein F
check En Stock
Hurry! only 10 items left in stock.
429,00 €
HT
Quantité

Ajouter souhaits

Product Information


Protein full name

SH2 domain-containing adapter protein F

Synonyms

Shf, SH2 domain-containing adapter protein F, Shb-like adapter protein, Src homology 2 domain containing F

Uniprot ID

Q8CG80

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Shf

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm



Applications


Applications Species Dilution Positive Tissue
IHC/IF Mouse, Rat 1: 300-1: 1200 brain, colon, lung



Background

Adapter protein which may play a role in the regulation of apoptosis in response to PDGF.



Images

012Du6ZsbAcHOD4.jpg

 IHC analysis of Shf(GB11688).
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02CNEDE42zmFwoC.jpg

 IHC analysis of Shf(GB11688).
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03I4jCwjMaZjnYE.jpg

 IF analysis of Shf(GB11688)red.
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

04KezLGmwRIo9gG.jpg

 IF analysis of Shf(GB11688)red.
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

05ChVgg7M9GmVJ2.jpg

 IF analysis of Shf(GB11688)red.
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB11688-100
: 10 Produits
Hurry! only 10 items left in stock.

Use collapsible tabs for more detailed information that will help customers make a purchasing decision.

Ex: Shipping and return policies, size guides, and other common questions.

  • Paste the label on a flat surface on the package
  • Make sure that both 1D and 2D barcodes are clearly visible
  • Ensure that the label is smooth and isn’t creased or wrinkled
  • Check for any tears, dents, holes or scratches
  • Pack your product tightly, with the right size packaging
  • Ensure both barcodes are on a flat surface of the package