Anti-Cytochrome c Mouse mAb
   

Anti-Cytochrome c Mouse mAb

Antibodies Oncology
AO-03-GB12080-100
GB12080,CC,12080,Cytochrome C,Cytochrome-C,Cytochrome c,somatic,GB12080100,cytochrome c,CYC,cyt-c,THC4,cytc,HCS,CytochromeC,cyt c,CYCS,Cytochrome c,细胞色素c,细胞色素,cyto-cytc,细胞色素c
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429,00 €
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Ajouter souhaits

Product Information


Protein full name

Cytochrome c

Synonyms

CYCS, CYC, HCS, THC4, cytochrome c, somatic, Cytochrome c, cyt c

Uniprot ID

P62897

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cytochrome C

Isotype

IgG1, κ

Clone Number

6B6D6

Predicted MW./Observed MW.

12 kDa / 14 kDa

Purity

Affinity purification

Subcellular location

Mitochondrion intermembrane space



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 500-1: 1000 heart
IHC/IF Human, Rat 1: 250-1: 1000 colon, stomach cancer, liver



Background

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase.Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol.



Images

01oKKJTSEjtLn6V.jpg

 WB analysis of Cytochrome C(GB12080).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

02x87QbJukABPd7.jpg

 IHC analysis of Cytochrome C(GB12080).
Sample: Human stomach cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

03PIjU9vEvWTqu2.jpg

 IHC analysis of Cytochrome C(GB12080).
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 6000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

04drhaiQKHF15IN.jpg

 IHC analysis of Cytochrome C(GB12080).
Sample: Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

05JAaH1DiqIzFtl.jpg

 IF analysis of Cytochrome C(GB12080)red.
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

06BPTJemnQXiulp.jpg

 IF analysis of Cytochrome C(GB12080)red.
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

07TvKniKycbZFgM.jpg

 IF analysis of Cytochrome C(GB12080)red.
Sample: Rat kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB12080-100
: 10 Produits
Hurry! only 10 items left in stock.

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