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Cleaved-Caspase-7 p20 (D198) rabbit pAb
AO-06-ES1023-50
Antibody Product Overview
| ELK.No | ES1023 |
| Product name | Cleaved-Caspase-7 p20 (D198) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | CASP7; MCH3; Caspase-7; CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; ICE-LAP3 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 840 |
| Human Swiss-Prot | P55210 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Caspase-7 |
| Fields | >>Apoptosis;>>Apoptosis - multiple species;>>TNF signaling pathway;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Pertussis;>>Legionellosis;>>Pathways in cancer;>>Lipid and atherosclerosis |
| Gene name | CASP7 |
| Protein name | Caspase7 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12369 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P97864 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 7. AA range:149-198 |
| Specificity | Cleaved-Caspase-7 p20 (D198) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-7 p20 protein resulting from cleavage adjacent to D198. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 20kD |
| Background | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012], |
| Function | catalytic activity:Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-.,enzyme regulation:Inhibited by isatin sulfonamides.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.,PTM:Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed |
| Subcellular location | Cytoplasm. |
| Expression | Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. |
Additional Images
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Western Blot analysis of various cells using Cleaved-Caspase-7 p20 (D198) Polyclonal Antibody
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Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24h, using Caspase 7 (p20,Cleaved-Asp198) Antibody. The lane on the right is blocked with the synthesized peptide.
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