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Antibody Product Overview
| ELK.No | ES10432 |
| Product name | UB2D1 rabbit pAb |
| Reactivity | Human;Rat;Mouse |
| Applications | WB;ELISA |
| Other name | |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7321 |
| Human Swiss-Prot | P51668 |
| Source | Rabbit |
| Isotype | IgG |
| Target | UB2D1 |
| Fields | >>Ubiquitin mediated proteolysis;>>Protein processing in endoplasmic reticulum;>>Shigellosis |
| Gene name | UBE2D1 SFT UBC5A UBCH5 UBCH5A |
| Protein name | Ubiquitin-conjugating enzyme E2 D1 (EC 6.3.2.19) (Stimulator of Fe transport) (SFT) (UBC4/5 homolog) (UbcH5) (Ubiquitin carrier protein D1) (Ubiquitin-conjugating enzyme E2(17)KB 1) (Ubiquitin-conjuga |
| Human gene link | |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P61080 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | D3ZDK2 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Specificity | UB2D1 Polyclonal Antibody detects endogenous levels of protein. |
| Formulation | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000 ELISA 1:5000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 16kD |
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011], |
| Function | catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,caution:PubMed:9362508 cloned and sequenced SFT which consisted of UBE2D1 last coding exon along with intronic sequences on the 5'-end of this exon. A function in iron transport has been described.,function:Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X.,tissue specificity:Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis., |
| Subcellular location | Cytoplasm . |
| Expression | Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis. |
Additional Images
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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES10432-50
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