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Antibody Product Overview
| ELK.No | ES10682 |
| Product name | DNJB2 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3300 |
| Human Swiss-Prot | P25686 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DNJB2 |
| Fields | >>Protein processing in endoplasmic reticulum |
| Gene name | DNAJB2 HSJ1 HSPF3 |
| Protein name | DnaJ homolog subfamily B member 2 (DnaJ protein homolog 1) (Heat shock 40 kDa protein 3) (Heat shock protein J1) (HSJ-1) |
| Human gene link | |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9QYI5 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 260-340 |
| Specificity | DNJB2 Polyclonal Antibody detects endogenous levels of protein. |
| Formulation | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000 ELISA 1:5000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 35kD |
| Background | This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011], |
| Function | alternative products:Experimental confirmation may be lacking for some isoforms,similarity:Contains 1 J domain.,similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats.,tissue specificity:Brain (neuronal layers). Weakly, in skeletal muscle and spleen., |
| Subcellular location | [Isoform 2]: Cytoplasm . Nucleus .; [Isoform 1]: Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side . |
| Expression | More abundantly expressed in neocortex, cerebellum, spinal cord and retina where it is expressed by neuronal cells (at protein level) (PubMed:1599432, PubMed:12754272). Detected at much lower level in non-neuronal tissues including kidney, lung, heart, skeletal muscle, spleen and testis (at protein level) (PubMed:12754272, PubMed:1599432). Isoform 1 is more abundant in neocortex and cerebellum compared to isoform 2 (at protein level) (PubMed:12754272). |
Additional Images
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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES10682-50
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