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Antibody Product Overview
| ELK.No | ES12456 |
| Product name | UBC12 rabbit pAb |
| Reactivity | Human; Mouse |
| Applications | WB |
| Other name | |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 9040 |
| Human Swiss-Prot | P61081 |
| Source | Rabbit |
| Isotype | IgG |
| Target | UBC12 |
| Fields | >>Ubiquitin mediated proteolysis |
| Gene name | UBE2M UBC12 |
| Protein name | UBC12 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 22192 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P61082 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from human UBC12 AA range: 37-87 |
| Specificity | This antibody detects endogenous levels of UBC12 at Human/Mouse |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1：500-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 20kD |
| Observed band (KD) | |
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.,domain:Both the N-terminal docking peptide and the catalytic core domain must bind the uba3-nae1 complex simultaneously for optimal transfer of nedd8.,function:Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase rbx1, but not rbx2, suggests that the rbx1-ube2m complex neddylates specific target proteins, such as cul1, cul2, cul3 and cul4. Involved in cell proliferation.,pathway:Protein modification; protein neddylation.,similarity:Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.,subunit:Interacts with UBA3 and RBX1., |
| Subcellular location | cytoplasm,cytosol,extracellular exosome, |
| Expression |
Additional Images
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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES12456-50
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