Français
English
C/EBP β (phospho Thr235) rabbit pAb
AO-06-ES1275-50
Antibody Product Overview
| ELK.No | ES1275 |
| Product name | C/EBP β (phospho Thr235) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CEBPB; LAP; TCF5; PP9092; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5; TCF-5 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 1051 |
| Human Swiss-Prot | P17676 |
| Source | Rabbit |
| Isotype | IgG |
| Target | C/EBP β |
| Fields | >>IL-17 signaling pathway;>>TNF signaling pathway;>>Tuberculosis;>>Transcriptional misregulation in cancer |
| Gene name | CEBPB |
| Protein name | CCAAT/enhancer-binding protein beta |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12608 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P28033 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24253 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P21272 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human C/EBP-beta around the phosphorylation site of Thr235/188. AA range:201-250 |
| Specificity | Phospho-C/EBP β (T235) Polyclonal Antibody detects endogenous levels of C/EBP β protein only when phosphorylated at T235. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 36kD |
| Background | This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013], |
| Function | function:Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'.,PTM:Sumoylated by polymeric chains of SUMO2 or SUMO3.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. C/EBP subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha, delta and gamma. Interacts with TRIM28 and PTGES2.,tissue specificity:Expressed at low levels in the lung, kidney and spleen., |
| Subcellular location | Nucleus . Cytoplasm . Translocates to the nucleus when phosphorylated at Ser-288. In T-cells when sumoylated drawn to pericentric heterochromatin thereby allowing proliferation (By similarity). . |
| Expression | Expressed at low levels in the lung, kidney and spleen. |
Additional Images
No image
Western Blot analysis of various cells using Phospho-C/EBP β (T235) Polyclonal Antibody diluted at 1:500
No image
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
No image
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C/EBP-beta (Phospho-Thr235/188) Antibody
No image
Immunofluorescence analysis of HepG2 cells, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES1275-50
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package