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DARPP-32 (phospho Thr75) rabbit pAb
AO-06-ES1297-50
Antibody Product Overview
| ELK.No | ES1297 |
| Product name | DARPP-32 (phospho Thr75) rabbit pAb |
| Reactivity | Human;Mouse;Rat;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | PPP1R1B; DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 84152 |
| Human Swiss-Prot | Q9UD71 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DARPP-32 |
| Fields | >>cAMP signaling pathway;>>Dopaminergic synapse;>>Cocaine addiction;>>Amphetamine addiction;>>Alcoholism |
| Gene name | PPP1R1B |
| Protein name | Protein phosphatase 1 regulatory subunit 1B |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 19049 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q60829 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 360616 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q6J4I0 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75. AA range:41-90 |
| Specificity | Phospho-DARPP-32 (T75) Polyclonal Antibody detects endogenous levels of DARPP-32 protein only when phosphorylated at T75. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 32kD |
| Background | This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011], |
| Function | function:Inhibitor of protein-phosphatase 1.,PTM:Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.,PTM:Phosphorylation of Thr-34 is required for activity.,similarity:Belongs to the protein phosphatase inhibitor 1 family., |
| Subcellular location | Cytoplasm. |
| Expression | Adipose tissue,Brain,Cerebellum,Colon,Ovary, |
Additional Images
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Western Blot analysis of cell lysis treated or untreated by LPS 100ng/mL 30min, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
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Western Blot analysis of various cells using Phospho-DARPP-32 (T75) Polyclonal Antibody diluted at 1:500
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
: AO-06-ES1297-50
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