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GSK3α/β (phospho Tyr279/216) rabbit pAb
AO-06-ES1321-50
Antibody Product Overview
| ELK.No | ES1321 |
| Product name | GSK3α/β (phospho Tyr279/216) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IF;WB;IHC;ELISA |
| Other name | GSK3A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 2931 |
| Human Swiss-Prot | P49840/P49841 |
| Source | Rabbit |
| Isotype | IgG |
| Target | GSK3α/β |
| Fields | >>Chemokine signaling pathway;>>Dopaminergic synapse;>>Non-alcoholic fatty liver disease;>>Shigellosis |
| Gene name | GSK3A/GSK3B |
| Protein name | Glycogen synthase kinase-3 alpha/beta |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 606496/56637 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | 50686/84027 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P18265/P18266 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216. AA range:246-295 |
| Specificity | Phospho-GSK3α/β (Y279/216) Polyclonal Antibody detects endogenous levels of GSK3α/β protein only when phosphorylated at Y279/216. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 51,46kD |
| Background | glycogen synthase kinase 3 alpha(GSK3A) Homo sapiens This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011], |
| Function | catalytic activity:ATP + [tau protein] = ADP + [tau protein] phosphate.,function:Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Monomer., |
| Subcellular location | mitochondrion,cytosol,beta-catenin destruction complex,postsynapse, |
| Expression | Brain,Eye,Foreskin,Lung,Pancreas,Platelet,Testis, |
Additional Images
No image
Immunofluorescence analysis of Hela cell. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
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Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
No image
Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α/β (phospho Tyr279/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
No image
Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
: AO-06-ES1321-50
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