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Jun B (phospho Ser259) rabbit pAb
AO-06-ES1351-50
Antibody Product Overview
| ELK.No | ES1351 |
| Product name | Jun B (phospho Ser259) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;IP;ELISA |
| Other name | JUNB; Transcription factor jun-B |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3726 |
| Human Swiss-Prot | P17275 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Jun B |
| Fields | >>Osteoclast differentiation;>>TNF signaling pathway;>>Growth hormone synthesis, secretion and action |
| Gene name | JUNB |
| Protein name | Transcription factor jun-B |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16477 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P09450 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24517 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P24898 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human JunB around the phosphorylation site of Ser259. AA range:226-275 |
| Specificity | Phospho-Jun B (S259) Polyclonal Antibody detects endogenous levels of Jun B protein only when phosphorylated at S259. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 33kD |
| Background | function:Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.,induction:By growth factors.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Jun subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as an homodimer or as an heterodimer with another member of the jun/fos family., |
| Function | function:Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.,induction:By growth factors.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Jun subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as an homodimer or as an heterodimer with another member of the jun/fos family., |
| Subcellular location | Nucleus. |
| Expression | Adipose tissue,Epithelium,Pancreas,Placenta,Prostat |
Additional Images
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Western Blot analysis of various cells using Phospho-Jun B (S259) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunB (Phospho-Ser259) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HeLa cells, using JunB (Phospho-Ser259) Antibody. The lane on the right is blocked with the phospho peptide.
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