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Rb (phospho Ser780) rabbit pAb
AO-06-ES1400-50
Antibody Product Overview
| ELK.No | ES1400 |
| Product name | Rb (phospho Ser780) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5925 |
| Human Swiss-Prot | P06400 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rb |
| Fields | >>Endocrine resistance;>>Cell cycle;>>Cellular senescence;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Chemical carcinogenesis - receptor activation;>>Pancreatic cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
| Gene name | RB1 |
| Protein name | Retinoblastoma-associated protein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 19645 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P13405 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24708 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P33568 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800 |
| Specificity | Phospho-Rb (S780) Polyclonal Antibody detects endogenous levels of Rb protein only when phosphorylated at S780. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 106kD |
| Background | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008], |
| Function | disease:Defects in RB1 are a cause of bladder cancer [MIM:109800].,disease:Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500].,disease:Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ("cat eye") is investigated.,function:Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts |
| Subcellular location | Nucleus . During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization. . |
| Expression | Expressed in the retina. Expressed in foreskin keratinocytes (at protein level) (PubMed:20940255). |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,Rb (phospho Ser780) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GFAP Monoclonal Antibody(5C8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
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Western Blot analysis of various cells using Phospho-Rb (S780) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western Blot analysis of KB cells using Phospho-Rb (S780) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Retinoblastoma (Phospho-Ser780) Antibody. The picture on the right is blocked with the phospho peptide.
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