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Hrs (phospho Tyr216) rabbit pAb
AO-06-ES1329-50
Antibody Product Overview
| ELK.No | ES1329 |
| Product name | Hrs (phospho Tyr216) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | HGS; HRS; Hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 9146 |
| Human Swiss-Prot | O14964 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Hrs |
| Fields | >>Viral life cycle - HIV-1;>>Endocytosis;>>Phagosome |
| Gene name | HGS |
| Protein name | Hepatocyte growth factor-regulated tyrosine kinase substrate |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15239 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q99LI8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 56084 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9JJ50 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Hrs (phospho Tyr216) |
| Specificity | Phospho-Hrs (Y216) Polyclonal Antibody detects endogenous levels of Hrs protein only when phosphorylated at Y216. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 115kD |
| Background | The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010], |
| Function | domain:Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.,function:Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the |
| Subcellular location | Cytoplasm . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome, multivesicular body membrane ; Peripheral membrane protein . Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments. . |
| Expression | Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. |
Additional Images
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Western Blot analysis of various cells using Phospho-Hrs (Y216) Polyclonal Antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Hrs (Phospho-Tyr216) Antibody
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Western blot analysis of lysates from A549 cells, using phospho-Hrs (Phospho-Tyr216) antibody.
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: AO-06-ES1329-50
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