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Antibody Product Overview
| ELK.No | ES1545 |
| Product name | 14-3-3 θ/τ rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 10971 |
| Human Swiss-Prot | P27348 |
| Source | Rabbit |
| Isotype | IgG |
| Target | 14-3-3 θ/τ |
| Fields | >>Cell cycle;>>Oocyte meiosis;>>PI3K-Akt signaling pathway;>>Hippo signaling pathway;>>Hepatitis C;>>Hepatitis B;>>Viral carcinogenesis |
| Gene name | YWHAQ |
| Protein name | 14-3-3 protein theta |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 22630 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P68254 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25577 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P68255 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau. AA range:196-245 |
| Specificity | 14-3-3 θ/τ Polyclonal Antibody detects endogenous levels of 14-3-3 θ/τ protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 28kD |
| Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008], |
| Function | function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,similarity:Belongs to the 14-3-3 family.,subcellular location:In neurons, axonally transported to the nerve terminals.,subunit:Homodimer. Interacts with PCTK1 (By similarity). Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm.,tissue specificity:Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in i |
| Subcellular location | Cytoplasm. In neurons, axonally transported to the nerve terminals. |
| Expression | Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. |
Additional Images
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Western Blot analysis of various cells using 14-3-3 θ/τ Polyclonal Antibody diluted at 1:500
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Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa cells, using 14-3-3 thet/tau Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES1545-100
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