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Antibody Product Overview
| ELK.No | ES15919 |
| Product name | GPX4 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB |
| Other name | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial (PHGPx) (EC 1.11.1.12) (Glutathione peroxidase 4) (GPx-4) (GSHPx-4) |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 2879 |
| Human Swiss-Prot | P36969 |
| Source | Rabbit |
| Isotype | IgG |
| Target | GPX4 |
| Fields | >>Glutathione metabolism;>>Metabolic pathways;>>Ferroptosis |
| Gene name | GPX4 |
| Protein name | GPX4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from human GPX4 AA range: 84-134 |
| Specificity | This antibody detects endogenous levels of GPX4 at Human |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 20kD |
| Background | The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016], |
| Function | catalytic activity:2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H(2)O.,disease:Defects in GPX4 may be a cause of infertility.,function:Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.,similarity:Belongs to the glutathione peroxidase family.,subunit:Monomer. Has a tendency to form higher mass oligomers.,tissue specificity:Present primarily in testis., |
| Subcellular location | [Isoform Mitochondrial]: Mitochondrion .; [Isoform Cytoplasmic]: Cytoplasm . |
| Expression | Present primarily in testis. Expressed in platelets (at protein level) (PubMed:11115402). |
Additional Images
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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES15919-50
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