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Antibody Product Overview
| ELK.No | ES1621 |
| Product name | AKAP 149 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | AKAP1; AKAP149; PRKA1; A-kinase anchor protein 1; mitochondrial; A-kinase anchor protein 149 kDa; AKAP 149; Dual specificity A-kinase-anchoring protein 1; D-AKAP-1; Protein kinase A-anchoring protein 1; PRKA1; Spermatid A-kinase anchor prot |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8165 |
| Human Swiss-Prot | Q92667 |
| Source | Rabbit |
| Isotype | IgG |
| Target | AKAP 149 |
| Fields | |
| Gene name | AKAP1 |
| Protein name | A-kinase anchor protein 1 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | O08715 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AKAP1. AA range:281-330 |
| Specificity | AKAP 149 Polyclonal Antibody detects endogenous levels of AKAP 149 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 90kD |
| Background | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008], |
| Function | domain:RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.,function:Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane.,similarity:Contains 1 KH domain.,similarity:Contains 1 Tudor domain.,tissue specificity:AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma., |
| Subcellular location | Mitochondrion outer membrane . Mitochondrion . |
| Expression | Isoform 1 is detected in thymus, prostate, testis, ovary, colon and small intestine (PubMed:8769136). Isoform 2 is highly expressed in testis and detected at much lower levels in kidney, pancreas, liver, lung and brain (PubMed:7499250). |
Additional Images
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Western Blot analysis of various cells using AKAP 149 Polyclonal Antibody diluted at 1:2000
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Immunofluorescence analysis of COS7 cells, using AKAP1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HUVEC and COLO cells, using AKAP1 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES1621-50
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