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Antibody Product Overview
| ELK.No | ES1644 |
| Product name | ALX3 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | ALX3; Homeobox protein aristaless-like 3; Proline-rich transcription factor ALX3 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 257 |
| Human Swiss-Prot | O95076 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ALX3 |
| Fields | |
| Gene name | ALX3 |
| Protein name | Homeobox protein aristaless-like 3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11694 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O70137 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ALX3. AA range:171-220 |
| Specificity | ALX3 Polyclonal Antibody detects endogenous levels of ALX3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 36kD |
| Background | This gene encodes a nuclear protein with a homeobox DNA-binding domain that functions as a transcriptional regulator involved in cell-type differentiation and development. Preferential methylation of this gene's promoter is associated with advanced-stage neuroblastoma tumors. [provided by RefSeq, Jul 2008], |
| Function | function:Transcriptional regulator with a possible role in patterning of mesoderm during development.,similarity:Belongs to the paired homeobox family.,similarity:Contains 1 homeobox DNA-binding domain., |
| Subcellular location | Nucleus . |
| Expression | Brain,Neuroblastoma, |
Additional Images
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Western Blot analysis of various cells using ALX3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of HeLa cells, using ALX3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from 293 cells, using ALX3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES1644-50
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