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Antibody Product Overview
| ELK.No | ES1688 |
| Product name | AQP2 rabbit pAb |
| Reactivity | Human;Mouse;Rat;Monkey;Dog |
| Applications | WB;IHC;IF;ELISA |
| Other name | AQP2; Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 359 |
| Human Swiss-Prot | P41181 |
| Source | Rabbit |
| Isotype | IgG |
| Target | AQP2 |
| Fields | >>Vasopressin-regulated water reabsorption |
| Gene name | AQP2 |
| Protein name | Aquaporin-2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11827 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P56402 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25386 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P34080 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Aquaporin 2. AA range:222-271 |
| Specificity | AQP2 Polyclonal Antibody detects endogenous levels of AQP2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 29kD |
| Background | This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008], |
| Function | disease:Defects in AQP2 are the cause of diabetes insipidus nephrogenic autosomal (ANDI) [MIM:125800]; also known as diabetes insipidus nephrogenic type 2. ANDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive.,domain:Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).,function:Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.,online information:AQP2 pages,PTM:Ser-256 phosphorylation is nec |
| Subcellular location | Apical cell membrane ; Multi-pass membrane protein . Basolateral cell membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane ; Multi-pass membrane protein . Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein . Shuttles from vesicles to the apical membrane (PubMed:15509592). Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane (PubMed:15509592). PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated (By similarity). . |
| Expression | Expressed in collecting tubules in kidney medulla (at protein level) (PubMed:7510718). Detected in kidney (PubMed:7510718). |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,AQP2 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:RS3608 was diluted at 1:1000(room temperature, 50min).
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Western Blot analysis of various cells using AQP2 Polyclonal Antibody
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Immunofluorescence analysis of HeLa cells, using Aquaporin 2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES1688-100
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