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Antibody Product Overview
| ELK.No | ES16951 |
| Product name | DHE3 rabbit pAb |
| Reactivity | Human; Mouse;Rat |
| Applications | WB |
| Other name | |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 2746 |
| Human Swiss-Prot | P00367 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DHE3 |
| Fields | >>Arginine biosynthesis;>>Alanine, aspartate and glutamate metabolism;>>Nitrogen metabolism;>>Metabolic pathways;>>Carbon metabolism;>>Necroptosis;>>Proximal tubule bicarbonate reclamation |
| Gene name | GLUD1 GLUD |
| Protein name | DHE3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 14661 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P26443 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24399 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P10860 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from human DHE3 |
| Specificity | This antibody detects endogenous levels of DHE3 at Human/Mouse/Rat |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1：500-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 61kD |
| Observed band (KD) | |
| Background | This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016], |
| Function | catalytic activity:L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H.,disease:Defects in GLUD1 are the cause of hyperinsulinism-hyperammonemia syndrome (HHS) [MIM:606762]. Elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.,enzyme regulation:Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme.,function:May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.,online information:Glutamate dehydrogenase 1 entry,similarity:Belongs to the Glu/Leu/Phe/Val dehydrogenases family.,subunit:Homohexamer., |
| Subcellular location | Mitochondrion . Endoplasmic reticulum . Mostly translocates into the mitochondria, only a small amount of the protein localizes to the endoplasmic reticulum. . |
| Expression |
Additional Images
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Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES16951-50
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