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Antibody Product Overview
| ELK.No | ES1738 |
| Product name | AW-1 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | ZNF174; ZSCAN8; Zinc finger protein 174; AW-1; Zinc finger and SCAN domain-containing protein 8 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7727 |
| Human Swiss-Prot | Q15697 |
| Source | Rabbit |
| Isotype | IgG |
| Target | AW-1 |
| Fields | |
| Gene name | ZNF174 |
| Protein name | Zinc finger protein 174 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ZNF174. AA range:201-250 |
| Specificity | AW-1 Polyclonal Antibody detects endogenous levels of AW-1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 46kD |
| Background | function:Transcriptional repressor.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 1 SCAN box domain.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Homodimer.,tissue specificity:Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and HEPG2 cells., |
| Function | function:Transcriptional repressor.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 1 SCAN box domain.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Homodimer.,tissue specificity:Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and HEPG2 cells., |
| Subcellular location | Nucleus. |
| Expression | Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and Hep-G2 cells. |
Additional Images
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Western Blot analysis of various cells using AW-1 Polyclonal Antibody
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Immunofluorescence analysis of HUVEC cells, using ZNF174 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa cells, using ZNF174 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES1738-50
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