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Antibody Product Overview
| ELK.No | ES1793 |
| Product name | Brp44L rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | MPC1; BRP44L; CGI-129; HSPC040; PNAS-115; Mitochondrial pyruvate carrier 1; Brain protein 44-like protein |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 51660 |
| Human Swiss-Prot | Q9Y5U8 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Brp44L |
| Fields | >>Diabetic cardiomyopathy |
| Gene name | MPC1 |
| Protein name | Mitochondrial pyruvate carrier 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 55951 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P63030 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 171087 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P63031 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BRP44L. AA range:11-60 |
| Specificity | Brp44L Polyclonal Antibody detects endogenous levels of Brp44L protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 12kD |
| Background | The protein encoded by this gene is part of an MPC1/MPC2 heterodimer that is responsible for transporting pyruvate into mitochondria. The encoded protein is found in the inner mitochondrial membrane. Defects in this gene are a cause of mitochondrial pyruvate carrier deficiency. Several transcript variants, some protein coding and one non-protein coding, have been found for this gene. [provided by RefSeq, Aug 2012], |
| Function | similarity:Belongs to the UPF0041 (0-44) family., |
| Subcellular location | Mitochondrion inner membrane ; Multi-pass membrane protein . |
| Expression | Placenta,Promyelocytic leukemia,Skeletal muscle,Umbilical cord blood, |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,Brp44L Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:RS3608 was diluted at 1:1000(room temperature, 50min).
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Western Blot analysis of various cells using Brp44L Polyclonal Antibody
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Immunofluorescence analysis of A549 cells, using BRP44L Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using BRP44L Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES1793-100
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