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AMPKα1 (phospho-Ser485) rabbit pAb
AO-06-ES18372-100
Antibody Product Overview
| ELK.No | ES18372 |
| Product name | AMPKα1 (phospho-Ser485) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB |
| Other name | 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1) (EC 2.7.11.1) (Acetyl-CoA carboxylase kinase) (ACACA kinase) (EC 2.7.11.27) (Hydroxymethylglutaryl-CoA reductase kinase) (HMGCR kinase) (EC 2.7.11.31) (Tau-protein kinase PRKAA1) (EC 2.7.11.26) |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5562 |
| Human Swiss-Prot | Q13131 |
| Source | Rabbit |
| Isotype | IgG |
| Target | AMPKα1 |
| Fields | >>FoxO signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Apelin signaling pathway;>>Tight junction;>>Circadian rhythm;>>Thermogenesis;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Oxytocin signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Alcoholic liver disease;>>Hypertrophic cardiomyopathy;>>Fluid shear stress and atherosclerosis |
| Gene name | PRKAA1 AMPK1 |
| Protein name | AMPKα1 (Ser485) |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 105787 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q5EG47 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 65248 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P54645 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized phosho peptide around human AMPKα1 (Ser485) |
| Specificity | This antibody detects endogenous levels of Human Mouse Rat AMPKα1 (phospho-Ser485) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:1000-2000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 65kD |
| Background | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic s |
| Subcellular location | Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters. . |
| Expression | Brain,Intestine,Liver,Mammary gland,Platelet,Testis |
Additional Images
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Western blot analysis of lysates from HeLa cells treated with heat shock, using AMPK1 (Phospho-Ser485) Antibody. The lane on the right is blocked with the phospho peptide.
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