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Antibody Product Overview
| ELK.No | ES2001 |
| Product name | CLK1 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | CLK1; CLK; Dual specificity protein kinase CLK1; CDC-like kinase 1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 1195 |
| Human Swiss-Prot | P49759 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CLK1 |
| Fields | >>Legionellosis |
| Gene name | CLK1 |
| Protein name | Dual specificity protein kinase CLK1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12747 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P22518 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CLK1. AA range:101-150 |
| Specificity | CLK1 Polyclonal Antibody detects endogenous levels of CLK1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 57kD |
| Background | CDC like kinase 1(CLK1) Homo sapiens This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines.,PTM:Autophosphorylates on all three types of residues.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PPIG., |
| Subcellular location | Nucleus. |
| Expression | Endothelial cells. |
Additional Images
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Western Blot analysis of various cells using CLK1 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of KB lysis using CLK1 antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of HUVEC cells, using CLK1 Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES2001-50
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