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Rac1/CDC42 (Phospho Ser71) rabbit pAb
AO-06-ES20202-100
Antibody Product Overview
| ELK.No | ES20202 |
| Product name | Rac1/CDC42 (Phospho Ser71) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | Ras-related C3 botulinum toxin substrate 1 (Cell migration-inducing gene 5 protein;Ras-like protein TC25;p21-Rac1) |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5879 |
| Human Swiss-Prot | P63000/P15153/P60763/P60953 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rac1/CDC42 |
| Fields | >>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>Sphingolipid signaling pathway;>>Phagosome;>>PI3K-Akt signaling pathway;>>Wnt signaling pathway;>>Axon guidance;>>VEGF signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>Natural killer cell mediated cytotoxicity;>>B cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Neurotrophin signaling pathway;>>Regulation of actin cytoskeleton;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>Pancreatic secretion;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Bacterial invasion of epithelial cells;>>Epithelial cell signaling in Helicobacter pylori infect |
| Gene name | RAC1 TC25 MIG5 |
| Protein name | Rac1/CDC42 (Phospho Ser71) |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 19353 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P63001 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 363875 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q6RUV5 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from human Rac1/CDC42 (Phospho Ser71) |
| Specificity | This antibody detects endogenous levels of Human,Mouse,Rat Rac1/CDC42 (Phospho Ser71) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 21kD |
| Observed band (KD) | |
| Background | |
| Function | |
| Subcellular location | Cell membrane ; Lipid-anchor ; Cytoplasmic side . Melanosome . Cytoplasm . Cell projection, lamellipodium . Cell projection, dendrite . Cell junction, synapse . Nucleus . Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (PubMed:1903399). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Localizes to the lamellipodium in a SH3RF1-dependent manner (By similarity). In macrophages, cytoplasmic location increases upon CSF1 stimulation (By similarity). Activation by GTP-binding promotes nuclear localization (PubMed:12551911). . |
| Expression | Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. |
Additional Images
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Western blot analysis of Rac1(Phospho-Ser71) antibody in 293 cells lysates, cell treated or untreated with PMA 125ng/ml 30', 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rac1(Phospho-Ser71) antibody
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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES20202-100
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