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Antibody Product Overview
| ELK.No | ES2086 |
| Product name | Cyclin H rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CCNH; Cyclin-H; MO15-associated protein; p34; p37 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 902 |
| Human Swiss-Prot | P51946 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Cyclin H |
| Fields | >>Basal transcription factors;>>Nucleotide excision repair;>>Cell cycle |
| Gene name | CCNH |
| Protein name | Cyclin-H |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 66671 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61458 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 84389 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9R1A0 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cyclin H. AA range:274-323 |
| Specificity | Cyclin H Polyclonal Antibody detects endogenous levels of Cyclin H protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 38kD |
| Background | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple t |
| Function | function:Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin C subfamily.,subunit:Associates primarily with CDK7 and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor., |
| Subcellular location | Nucleus. |
| Expression | Bone marrow,Brain,Embryonic brain,Epithelium,Liver,Urinary bladder, |
Additional Images
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Western Blot analysis of various cells using Cyclin H Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of HeLa cells, using Cyclin H Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Cyclin H Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HepG2, LOVO, and RAW264.7 cells, using Cyclin H Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES2086-50
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