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Antibody Product Overview
| ELK.No | ES2118 |
| Product name | Cystatin A rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | CSTA; STF1; STFA; Cystatin-A; Cystatin-AS; Stefin-A |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1475 |
| Human Swiss-Prot | P01040 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Cystatin A |
| Fields | |
| Gene name | CSTA |
| Protein name | Cystatin-A |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P56567 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Stefin A. AA range:49-98 |
| Specificity | Cystatin A Polyclonal Antibody detects endogenous levels of Cystatin A protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 11kD |
| Background | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008], |
| Function | function:This is an intracellular thiol proteinase inhibitor.,similarity:Belongs to the cystatin family., |
| Subcellular location | Cytoplasm . |
| Expression | Expressed in the skin throughout the epidermis. |
Additional Images
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Western Blot analysis of various cells using Cystatin A Polyclonal Antibody
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Western Blot analysis of HT29 cells using Cystatin A Polyclonal Antibody
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Immunofluorescence analysis of A549 cells, using Stefin A Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Stefin A Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES2118-100
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