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Antibody Product Overview
| ELK.No | ES2160 |
| Product name | DDX8 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | DHX8; DDX8; ATP-dependent RNA helicase DHX8; DEAH box protein 8; RNA helicase HRH1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 1659 |
| Human Swiss-Prot | Q14562 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DDX8 |
| Fields | >>Spliceosome |
| Gene name | DHX8 |
| Protein name | ATP-dependent RNA helicase DHX8 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 217207 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | A2A4P0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DHX8. AA range:391-440 |
| Specificity | DDX8 Polyclonal Antibody detects endogenous levels of DDX8 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 150kD |
| Background | This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014], |
| Function | domain:The RS domain confers a nuclear localization signal, and appears to facilitate the interaction with the spliceosome.,function:Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.,similarity:Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 1 S1 motif domain.,subunit:Identified in the spliceosome C complex, at least composed of AQR, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3 |
| Subcellular location | Nucleus . |
| Expression | Expressed in skin, cervix and nerve. Also expressed in the brain. |
Additional Images
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Western Blot analysis of various cells using DDX8 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of the lysates from HeLa cells using DHX8 antibody.
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: AO-06-ES2160-50
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