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Antibody Product Overview
| ELK.No | ES2184 |
| Product name | DNA pol α rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | POLA1; POLA; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5422 |
| Human Swiss-Prot | P09884 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DNA pol α |
| Fields | >>DNA replication |
| Gene name | POLA1 |
| Protein name | DNA polymerase alpha catalytic subunit |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P33609 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DNA Polymerase alpha. AA range:81-130 |
| Specificity | DNA pol α Polyclonal Antibody detects endogenous levels of DNA pol α protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 165kD |
| Background | This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010], |
| Function | catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Polymerase alpha in a complex with DNA primase is a replicative polymerase.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,PTM:A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.,similarity:Belongs to the DNA polymerase type-B family.,subunit:Interacts with SV40 Large T antigen; this interaction allows viral DNA replication., |
| Subcellular location | Nucleus . Cytoplasm, cytosol . In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern. . |
| Expression |
Additional Images
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Western Blot analysis of various cells using DNA pol α Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from HepG2, 293, and Jurkat cells, using DNA Polymerase alpha Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES2184-50
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