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Antibody Product Overview
| ELK.No | ES2196 |
| Product name | DPF2 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | DPF2; BAF45D; REQ; UBID4; Zinc finger protein ubi-d4; Apoptosis response zinc finger protein; BRG1-associated factor 45D; BAF45D; D4; zinc and double PHD fingers family 2; Protein requiem |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5977 |
| Human Swiss-Prot | Q92785 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DPF2 |
| Fields | |
| Gene name | DPF2 |
| Protein name | Zinc finger protein ubi-d4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 19708 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61103 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human REQU. AA range:151-200 |
| Specificity | DPF2 Polyclonal Antibody detects endogenous levels of DPF2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 44kD |
| Background | The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008], |
| Function | function:May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.,similarity:Belongs to the requiem/DPF family.,similarity:Contains 1 C2H2-type zinc finger.,similarity:Contains 2 PHD-type zinc fingers.,subcellular location:30% nuclear. 70% cytoplasmic.,tissue specificity:Ubiquitous., |
| Subcellular location | Nucleus . Cytoplasm . |
| Expression | Ubiquitous. |
Additional Images
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Western Blot analysis of various cells using DPF2 Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of A549 cells, using REQU Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HT-29 cells, using REQU Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES2196-50
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