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Antibody Product Overview
| ELK.No | ES2494 |
| Product name | HBO1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IF;ELISA |
| Other name | KAT7; HBO1; HBOa; MYST2; Histone acetyltransferase KAT7; Histone acetyltransferase binding to ORC1; Lysine acetyltransferase 7; MOZ; YBF2/SAS3, SAS2 and TIP60 protein 2; MYST-2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 11143 |
| Human Swiss-Prot | O95251 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HBO1 |
| Fields | |
| Gene name | KAT7 |
| Protein name | Histone acetyltransferase KAT7 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 217127 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q5SVQ0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 303470 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q810T5 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MYST2. AA range:131-180 |
| Specificity | HBO1 Polyclonal Antibody detects endogenous levels of HBO1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 75kD |
| Background | catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone.,domain:The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.,domain:The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.,function:Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MYST (SAS/MOZ) family.,similarity:Contains 1 C2HC-type zinc finger.,subunit:Component of the HBO1 complex composed at least of ING4 or ING5, MYTS2/HBO1, EAF6, and one of PHF15, PHF16 and PHF17. Interacts with MCM2 and ORC1L. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone.,tissue specificity:Ubiquitously expressed, with highest levels in testis., |
| Function | catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone.,domain:The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.,domain:The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.,function:Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MYST (SAS/MOZ) family.,similarity:Contains 1 C2HC-type zinc finger.,subunit:Component of the HBO1 complex composed at least of ING4 or ING5, MYTS2/HBO1, EAF6, and one of PHF15, PHF16 an |
| Subcellular location | Nucleus . Chromosome . Chromosome, centromere . Cytoplasm, cytosol . Associates with replication origins specifically during the G1 phase of the cell cycle (PubMed:18832067, PubMed:20129055). Localizes to transcription start sites (PubMed:21753189, PubMed:24065767). Localizes to ultraviolet-induced DNA damage sites following phosphorylation by ATR (PubMed:28719581). Localizes to centromeres in G1 phase (PubMed:27270040). . |
| Expression | Ubiquitously expressed, with highest levels in testis. |
Additional Images
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Western Blot analysis of various cells using HBO1 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western Blot analysis of Jurkat cells using HBO1 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of HUVEC cells, using MYST2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using MYST2 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES2494-50
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