Français
English
Antibody Product Overview
| ELK.No | ES2515 |
| Product name | HisRS rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | HARS; HRS; Histidine--tRNA ligase; cytoplasmic; Histidyl-tRNA synthetase; HisRS |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3035 |
| Human Swiss-Prot | P12081 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HisRS |
| Fields | >>Aminoacyl-tRNA biosynthesis |
| Gene name | HARS |
| Protein name | Histidine--tRNA ligase cytoplasmic |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15115 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61035 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HARS. AA range:460-509 |
| Specificity | HisRS Polyclonal Antibody detects endogenous levels of HisRS protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 60kD |
| Background | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012], |
| Function | catalytic activity:ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).,similarity:Belongs to the class-II aminoacyl-tRNA synthetase family.,similarity:Contains 1 WHEP-TRS domain.,tissue specificity:Brain, heart, liver and kidney., |
| Subcellular location | Cytoplasm . |
| Expression | Brain, heart, liver and kidney. |
Additional Images
No image
Western Blot analysis of various cells using HisRS Polyclonal Antibody diluted at 1:2000
No image
Immunofluorescence analysis of HepG2 and HeLa cells, using HARS Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Western blot analysis of lysates from COLO cells, using HARS Antibody. The lane on the right is blocked with the synthesized peptide.
No image
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES2515-50
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package