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Antibody Product Overview
| ELK.No | ES2538 |
| Product name | HLX1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HLX; HLX1; H2.0-like homeobox protein; Homeobox protein HB24; Homeobox protein HLX1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3142 |
| Human Swiss-Prot | Q14774 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HLX1 |
| Fields | |
| Gene name | HLX |
| Protein name | H2.0-like homeobox protein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15284 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61670 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 364069 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | A0JPN1 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HLX1. AA range:321-370 |
| Specificity | HLX1 Polyclonal Antibody detects endogenous levels of HLX1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 75kD |
| Background | function:Transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis.,sequence caution:Translation N-terminally extended.,similarity:Belongs to the H2.0 homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,tissue specificity:Low level in normal B and T-cells, high level in activated lymphocytes and monocytes. Also found in thymus, tonsil, bone marrow, developing vessels, and fetal brain., |
| Function | function:Transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis.,sequence caution:Translation N-terminally extended.,similarity:Belongs to the H2.0 homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,tissue specificity:Low level in normal B and T-cells, high level in activated lymphocytes and monocytes. Also found in thymus, tonsil, bone marrow, developing vessels, and fetal brain., |
| Subcellular location | Nucleus . |
| Expression | Low level in normal B and T-cells, high level in activated lymphocytes and monocytes. Also found in thymus, tonsil, bone marrow, developing vessels, and fetal brain. |
Additional Images
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Western Blot analysis of various cells using HLX1 Polyclonal Antibody diluted at 1:1000
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of MCF7 cells, using HLX1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO205 cells, using HLX1 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES2538-50
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