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Antibody Product Overview
| ELK.No | ES2544 |
| Product name | hnRNP A1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HNRNPA1; HNRPA1; Heterogeneous nuclear ribonucleoprotein A1; hnRNP A1; Helix-destabilizing protein; Single-strand RNA-binding protein; hnRNP core protein A1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3178 |
| Human Swiss-Prot | P09651 |
| Source | Rabbit |
| Isotype | IgG |
| Target | hnRNP A1 |
| Fields | >>Spliceosome;>>Amyotrophic lateral sclerosis |
| Gene name | HNRNPA1 |
| Protein name | Heterogeneous nuclear ribonucleoprotein A1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15382 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P49312 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 29578 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P04256 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human hnRNP A1. AA range:51-100 |
| Specificity | hnRNP A1 Polyclonal Antibody detects endogenous levels of hnRNP A1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 33kD |
| Background | This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome. [provided by RefSeq, Feb 2016], |
| Function | function:Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection.,PTM:Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.,similarity:Contains 2 RRM (RNA recognition motif) domains.,subcellular location:Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes.,subunit:Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, S |
| Subcellular location | Nucleus . Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes (PubMed:17289661). .; Cytoplasm . (Microbial infection) In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner. .; Nucleus . (Microbial infection) SARS coronavirus-2/SARS-CoV-2 ORF6 protein increases accumulation to the nucleus. . |
| Expression | Bone marrow,Cervix,Cervix carcinoma,Epithelium,Eye,Fetal br |
Additional Images
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Western Blot analysis of various cells using hnRNP A1 Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of COLO205 cells using hnRNP A1 Polyclonal Antibody diluted at 1:2000
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Immunofluorescence analysis of HeLa cells, using hnRNP A1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using hnRNP A1 Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES2544-50
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